The Effect of Short Term Treatment with Ivermectin on the Oxidative Stress Parameters in the Tissues of Clarias Gariepinus (Burchell, 1822), Juvenile

Abstract

The effect of short term treatments with ivermectin on the oxidative stress parameters in the tissues of Clarias gariepinus juveniles was studied between July and September 2015. Three hundred healthy juveniles of fresh water African catfish, Clarias gariepinus were used for the study. The present study investigated the behavioural responses, lethal concentration of ivermectin and the effect of sublethal concentrations on lipid peroxidation , antioxidant parameters and acute toxicity in the tissue of C. gariepinus. All animals were divided into 7 groups: Gp 1 (Tap water) served as the untreated control (0) while groups 2- 7 were treated with graded levels of ivermetin 0.15, 0.30, 0.45, 0.60, 0.75, 0.90mg/l body weight and was done for 28 days. The percentage mortality increased as the concentrations increased. The 24, 48, 72 and 96h Lc50 of ivermectin were: 0.75, 0.62, 0.45, 0.38mg/L respectively indicating four groups. The tissues of fish were exposed to various sublethal levels of ivermectin. Catalase, lipid peroxidation, glutathione peroxidase and glutathione reductase, superoxide dismutase activities of liver, and gill was highest in the control when compared to other groups.