Abstract:
Sputa from 300 p a t i e n t s with chronic chest i n f e c t i o n s were
examined microscopically and analyzed b a c t e r i o l o g i c a l l y , Cellforms
morphalagically resemblxg those of aerobic actdnamycetes
were obscrvod, end i s o l a t e s were i d e n t i f i e d through convantionzl
tests, E x t r & - c e l l u l a r p r o t e i n s from cell-froo c u l t u r a f i l t r a
of h!ocardia a s t u r o i d u s were p u r i f i e d by u l t r o f i l t r a t i o n , and
otod through fsooluctric focussing 2nd "Wcstorn Blot ti,7g1'.
Nocrrrdia ast~.refdosa nkig~msf n these p r o t e i n s were i d o n t i f i e
through cnzyme-linked immunoassny. Immunoblot s t r i p s develop with those anigons were tested for specificity. The effect of specific antibodies and iron on the growth and verulanca of Nocerdio ostcroidos at its various growth phases were investigated in culture media and mice
